| Summary: | 碩士 === 國立清華大學 === 化學工程學系 === 103 === The development of biodegradable materials with elastomeric properties had become one of the most popular research topics in the past decade, and the need to produce new elastomeric polymers in large scale for a wide variety of applications had been ever increasing. Poly(glycerol sebacate) (PGS) is a novel elastomer, containing very good biocompatibility and biodegradability, has been applied to soft tissue regeneration to regenerate arteries. However, the fabrication of PGS requires high temperature and low pressure which limit its application in medicine and tissue engineering. Here, we report on the synthesis of PGS-based photocurable biodegradable polymer, poly(glycerol sebacate) acrylate (PGSA). FT-IR and NMR analyses were employed for the confirmation of successful acylation and ssNMR were employed for to establish a full synthesis protocol along with the purification protocol. The behavior of crosslinking density was examined by testing the thermal properties using DSC. A wide range of mechanical properties are obtained with respect to their Young’s modulus from 0.12 to 3.17 MPa, ultimate tensile strength between 0.1 and 1.2 MPa and strain to failure from 121% to 39% by changing the degree of acrylation. Linearly degradation properties are observed and are degraded 28-8.5% in 30 days when increasing the degree of acrylation from 15% to 60%. The slightly hydrophilic properties of various forms of PGSA were confirmed by contact angle test. A series of cell culture were conducted for the confirmation of purification protocol and cell preference on the different PGSA products. Post treatment of photocured PGSA was introduced to further crosslink PGSA to increase the mechanical properties while facilitating degradation. Meanwhile, a great improvement of cell adhesion was found. PGSA was applied in 3D printing by two photon polymerization technology. Though the development of this material is still in the early stage, it is believed that they possess great potential in the applications toward
regenerative medicine.
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