Sugar-Bound Dinitrosyl Iron Complex (DNIC)

• Main Authors: Chou, Che Fang, 周哲芳
• Other Authors: Liaw, Wen Feng
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/66065631747747445102

碩士 === 國立清華大學 === 化學系 === 103 === Abstract 　　Hepatocellular carcinoma (HCC), also called Liver cancer, is the sixth most common cancer worldwide and the leading cause of death amongst cirrhotic patients. Since there are asialoglycoprotein receptors, ASGP-R, in the surface of livers, ASGP-R can identify the structure of galactose. In this study, We tried to synthesize a lactose-bound compound, in order to drive the cancer-therapy compound into liver cells easily. Transformation of dinitrosyl iron complex (DNIC) [ Fe(CO)2(NO)2 ] into{Fe(NO)2}9-{Fe(NO)2}9 [Fe(μ-S-C2- Lactose)(NO)2 ]2 ( Sugar RRE ), triggered by Lactose-C2-Disulfide. The water solubility of Sugar RRE makes it difficult to get single-crystal X-ray structure, so the structure of Sugar RRE was validated by mass spectrum, infrared spectrum, UV-visible spectrometer and electron paramagnetic resonance (EPR).In series of assay, human liver cancer cell (HePG-2) was adopted as an experimental model . We expected that the binding of Sugar-RRE to ASGP-R is sospecific that RRE can penetrate into liver cancer cells easily, converted into DNICs and then release NO to kill liver cancer cells.