| Summary: | 碩士 === 國立臺南大學 === 生物科技學系碩士班 === 103 === Cancer is one of the major research subjects worldwide and is the leading cause of death in Taiwan for 33 years. Cancer can be treated by surgery, radiation, or chemotherapeutic agents. However, certain anticancer drugs can result in drug resistant tumors. Recent studies have demonstrated that a RNA-binding protein can affect cancer cell developing drug resistance. This protein is called HuR (Hu antigen R). Another studies have demonstrated that antimicrobial peptides (AMP) can affect cancer cell proliferation and antitumor therapy. In the present study, we investigated the role of HuR or AMP in mediating cytotoxic effect of epirubicin (Epi) in colon adenocarcinoma SW620 cells, the study using siRNA to knockdown HuR (siHuR) and overRNA to overexpress HuR (HuR) in colon adenocarcinoma SW620 cells. The results showed that siHuR or AMP promoted the pro-apoptotic effect of Epi by increasing reactive oxygen species. On the other hand, HA-HuR abolished such effect. The siHuR or AMP decreased the multiple drug resistance (MDR) genes of Epi treated SW620 cells. On the other hand, HA-HuR abolished such effect. These results indicate that siHuR or AMP may function as a MDR modulator to enhance anticancer efficacy of Epi.
Therefore, siHuR or AMP may have the benefit to reduce drug side effects by lowering dosage of anticancer drugs. This could have strong potential in developing innovative therapeutic strategy combating cancer.
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