| Summary: | 碩士 === 國立臺灣師範大學 === 化學系 === 103 === The objective of this thesis is to understand the effect of proteins on Nanodiamond. In this thesis two kinds of Nanodiamonds with different surface charge were used, reacting with four kinds of proteins respectively. Proteins used here are Myoglobin，Bovine serum albumin，Alpha-Lactalbumin and Insulin. By the assist of UV-Vis spectrometer, particle size analysis and MALDI, the surface coverage of proteins on Nanodiamonds can be calculated. Thus, the adsorption of Protein on Nanodiamonds can be observed.
Mixing two kinds of Nanodiamonds and four kinds of proteins gives eight kinds of results. These results can be briefly cataloged into three models, Equilibrium model, cluster model and polymer model. Equilibrium model means the status of protein adsorbed on the surface of Nanodiamond is a kinetic equilibrium. The equilibrium constant can be estimated and the affinity can be observed. The example is Myoglobin. Cluster model shows stronger adsorption of protein on Nanodiamond than Equilibrium model. The protein will adsorb on the Nanodiamond till the surface of Nanodaimond is used out. The example is Bovine serum albumin. Polymer model is similar to cluster model. The protein not only cover the surface of Nanodaimond, but form polymer with itself and form a shield on the Nanodiamond surface with more than one unit thick.
Key Words: Nanodiamond, Protein
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