| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 103 === 5-Fluorouracil (5-FU) is widely used in the treatment of cancer, and fucoidan can function as an adjuvant to regulate immune cells such as enhancing cell maturation and inducing immune responses. In this study, we developed 5-FU coated chitosan/fucoidan (CS/F) functional nanoparticles (NPs), which are expected as a desired treatment to combine immunotherapy with chemotherapy for cancer treatment. As the CS to F weight ratio was 5:1, the NP shows the best performance. Its diameter was 151.19 ± 9.06 nm, the zeta potential was 28.11 ± 3.01 mV and PDI was 0.22 ± 0.03. The encapsulation efficiency of 5-FU in CS/F NPs was 73.84 ± 0.83%, the loading capacity was 5.03 ± 0.05%. We observe in vitro drug release by modifying the weight ratio of polymer (CS and F) to 5-FU. As the polymer ratio increases, the release rate and amount of 5-FU are decreased; as 5-FU ratio increases, 5-FU is burst released from NPs and unable to maintain a long time. The 5-FU coated CF/S NPs have a strong toxic effect on colon carcinoma cell line HT-29 cells and can inducing HT-29 cells apoptosis. Furthermore, HT-29 cellular uptake of NPs was observed by confocal laser scanning transmission electron microscope. However, The 5-FU coated CF/S NPs showed no toxic effects on mouse fibroblasts cell line L929. In addition, the 5-FU coated CF/S NPs can induce TNF-α and GM-CSF secretion of macrophage (RAW264.7), and regulate the immune response promptly. According to the results, the 5-FU coated CF/S NPs are potential cancer therapy agents and expected as a desired treatment combining immunotherapy with chemotherapy to treat cancer.
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