A Study of Liquid Flow Parameters to Influence the Portable Biochip System DNA Detection with Magnetic Beads and Fluorescence

• Main Authors: Tai, Po-Chen, 戴伯臻
• Other Authors: Chang, Chung-Cheng
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/mdx6u8

碩士 === 國立臺灣海洋大學 === 電機工程學系 === 103 === In this paper, MEMs (Micro Electro Mechanical Systems) technology and computer aided software are used to prepare micro channel PDMS bio-chip and laser magnetic bead integrated detection system. Moreover, the electrical signal, impedance signal and speed analysis of microbial flushing are performed on the micro channel biological hybridization. In the chip preparation part, photo resist SU-8 GM1075 and FH6400 is used to prepare the micro channel chip mold using photo-lithography process technology and sputtering method. Moreover, casting technique is used for molding, sputtering is used for sputtering electrode Ti to be connected to glass substrate with platinum, finally, biological chip of this thesis is then prepared. In the system architecture preparation, green laser of wavelength 532nm is used, and high pass filter of wavelength 550nm is also used. Meanwhile, AutoCAD 2D/3D and PSoC are also used to prepare experiment system circuit and to make experimental platform for test system. In the microbial culturing aspect, edwardsiella and coli bacillus of different fragments are adopted to perform biological hybridization comparison. In the test aspect, this experiment contains fluorescence speed test, which is used to analyze the electrical signal of microbe hybridization, and magnetic bead speed test, which is used to analyze the impedance signal of microbe. Through these two types of test analysis experiments, and through the specificity on microbe from fluorescence probe HEX and magnetic bead probe USPIO-101, then electrical signal and impedance signal can be used to distinguish whether the microbial hybridization is a success or not. Meanwhile, this experiment not only reduces the test time, it also greatly reduces the cost of the test system, moreover, it is very easy to carry, therefore, in the future, it is expected to have wide application in bio-medical test area. Key words: DNA hybridization, Laser Induced Fluorescence, inter-digital electrode, PDMS, SU-8 micro channel, magnetic bead