| Summary: | 碩士 === 國立臺東大學 === 生命科學系碩士班 === 103 ===
The process of ethanol metabolism in the body will cause redox reaction of the hepatic cells to lose balance and produce grave amount of free radicals, proceeding to cause hepatic lipid accumulation and inflammation resulting in liver injury. The Monascus-fermented product and its secondary metabolites have many functional effects. Monascus-fermented red mold rice can raise antioxidant enzyme activities in the liver and restrain anti-inflammatory cytokines activities, which can protect the liver against the damage of alcohol. Monascin (MS) and ankaflavin (AK) not only performed anti-inflammatory effect but also had the effect of regulating body fat and blood lipids. Furthermore, dimerumic acid (DMA) and deferricoprogen (DFC) were also proven as the potent antioxidants. However, the functional compounds of Monascus for preventing alcohol liver disease still remains unclear in current. Therefore, the first part of this research reveals the abilities of reducing alcohol liver disease of Monascus-produced antioxidants DMA and DFC under the same concentration. The second part is to study effect of the effective dose of monascin (MS) and ankaflavin (AK) on the prevention of alcohol liver disease. In both experiments, the C57BL/6J mice were fed Lieber-DeCarli liquid diet for six weeks in order to establish an animal model of alcoholic liver disease. During the stage, the test substances were orally administrated to the ALD mice in order to investigate the prevention effect and the mechanism against ALD.The results state that DMA, DFC, MS and AK all lowered the liver weight/body weight ratio, and visibly decreased liver function indexes such as aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) of serum, however, DFC and AK had more significant lowering effect. The serum triglyceride (TG) and liver total cholesterol (TC) and TG all decreased through feeding the four test substances, in which MS and AK had more effect. The pathological statin proves that low dosage of MS and AK prevented the lipid deposition in the liver. Furthermore, DMA, DFC, MS and AK effectively increased the antioxidative enzyme activities and lower lipid peroxidation. However, AK performed the best antioxidative effect. According to these findings, AK is more effective on the prevention of alcoholic liver disease and fatty liver. Regarding the mechanism of alcoholic liver disease prevention, the DMA and DFC repressed the phosphorylation of mitogen-activated protein kinases (MAPK) family, and further reduced the eypression of the inflammatory-related proteins including tumor necrosis factor-
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