臺東地區溫泉嗜熱/鹽菌酵素活性篩選與 Anoxybacillus flavithermus HY-TTH-D23 熱穩定半乳糖苷酶特性分析

• Main Authors: Cheng-Chia Shih, 施政嘉
• Other Authors: Chih-Hui Lin
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/vmqw9n

碩士 === 國立臺東大學 === 生命科學系碩士班 === 103 === Thermophiles have evolved distinct characteristic such as thermozyme system to adapt high temperature environment. There are many thermozymes from thermophiles have applied to industrial processes such as food, starch processing and drug development. The major habitat of thermophiles are geothermal environment. There are several undisturbed hot-springs which are ideal thermophile and halophile study sites in Taitung. In the study, five study sites including JhaoRih, JinLun, HongYe, ChiXia and YouZaiHu were investigated. One hundred and ten of bacteria strains were isolated and identified in our study. The results of 16S rRNA gene sequence analysis showed that at least 12 new species of bacteria were isolated. Thermus sp. and Geobacillus sp. were the main species in T group. Anoxybacillus sp. was the main species in TTH group. Bacillus sp. was the main species in HM group. None of species of bacteria was dominate in HT group. The result of API ZYM® analysis showed that the thermophiles have more diverse enzyme activities than halophiles. Enzyme activity of lipase, phosphatase and glycosidase were common in thermophilic isolates, while protease was the most common enzyme in halophilic isolates. Among the bacteria isolates, the thermophilic Anoxybacillus flavithermus HY-TTH-D23 showed multiple promising enzyme activities includes leucine arylamidase,