| Summary: | 碩士 === 國立臺灣大學 === 生化科技學系 === 103 === In February 2013, a human-infecting avian influenza virus subtype H7N9 was first reported in China. It was reasserted from 3 low-pathogenicity avian influenza (LPAI) H7N3, H7N9 and H9N2. While the novel H7N9 causes mild illness in birds, most human cases showed rapidly progressive pneumonia, severe respiratory illness and even high mortality. Thus, research for profound understanding of the highly pathogenic avian influenza (HPAI) virus H7N9 as well as an accurate detection method for this virus is important for disease control in Taiwan. Hemagglutinin (HA) is a surface glycoprotein with high specificity and high antigenicity, which is suitable for the development an early diagnosis platform with monoclonal antibody (mAb) against the novel H7N9 viral proteins. In this study, 3 peptides which showed high immunogenicity and high specificity were synthesized for mice immunization. After 5 boosts, mice with efficient immune response were sacrificed to produces monoclonal antibodies (mAb). The hybridoma strains were first screened by enzyme-linked immunosorbent assay (ELISA) and then tested by western blot using H7N9 HA1 and H6N1 HA1 as antigens, respectively. Two mAb that are highly specific to 2 different epitopes of H7N9 HA1 were isolated. They will be used in developing the blocking ELISA and antigen-capture ELISA, as well as in clinical diagnosis and quick viral detection in the field.
|
|---|
