| Summary: | 碩士 === 國立臺灣大學 === 應用物理所 === 103 === Indocyanine green (ICG) is a near-infrared fluorescent dye which absorption peak about 780 nm and emission peak about 805 nm. ICG has been used for decades on medical and bio-image field. For example, doctor diagnose a patient’s hepatic function by injecting ICG into the patient and monitor its excretion with time. Since ICG is processed by hepatocytes from portal vein then excreted into the bile duct, diagnosis can be performed by drawing blood from the patient 15 minutes following ICG injection. In this study, we studied ICG metabolism within the liver acinus which is classified into three zones: zone I, zone II, and zone III. Epi-illuminated confocal fluorescence microscope is used with a liver imaging chamber to observe ICG metabolism in vivo. By analyzing ICG liver images found that ICG fluorescence was degraded by excitation light. We discussed ICG excretion rate of hepatocytes to proceed qualitative analysis in three zones. Further, studying nucleus and cytoplasm of individual hepatocyte separately because of the image contrast between nucleus and cytoplasm. In an acinus, zone I hepatocytes excrete ICG fastest. Nucleus excretes ICG fast than cytoplasm independent of zone classified.
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