Water Extract of Gastrodia elata Bl. May Have Antidepressant Effect via Enhancing Neuroplasticity in Chronic Mild Stress Model

• Main Authors: YING-YU CHOU, 周映妤
• Other Authors: 沈立言
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/48469790254451412054

碩士 === 國立臺灣大學 === 食品科技研究所 === 103 === According to the World Health Organization（WHO） statistics in 2002, major depressive disorder was defined as one of the three major diseases in 21th century and over 87% suicide victims has mood disorder. In Taiwan, there is over 8.9% population has the depression. Therefore, it is necessary for investigative the pathophysiology of depression and the antidepressant therapeutics. However, depression pathology is multifactorial, which includes monoamine dysregulation, HPA（Hypothalamic–pituitary–adrenal）dysfunction, chronic inflammation and neuroplasticity reduction. The antidepressant used in clinics now brings a lot of side effects. Thus, looking for the alternative therapy and prevention strategy for depression are very important issues. One of animal models used to study depression is the chronic mild stress model .It is a well-known animal model which has high-validity, mimics the real human stressed environment. Recently, Gastrodia elata Blume has shown anxiolytic, mood and memory enhancing effects. In previous study, the water extract of Gastrodia elata Blume (WGE) has antidepressant-like effects in the forced swimming model. Therefore, the aim of this project is to investigate the antidepressant effects of Gastrodia elata Blume in the chronic mild stress model. The results of behavior showed that CMS group markedly decreased in sucrose intake in week 4 comparing to normal group. The group of WGE medium dose and low dose had significantly different with CMS group separately in week 5 and week 6. In the open field test, CMS showed significantly lower than normal and WGE treatment group in crossing in week 4 and week 5. In the rearing part, CMS also had significantly different with normal in week 4 to week 6, even with fluoxetine and L-WGE in week 6. The result of IHC (immunohistochemistry)showed that CMS group was lower number of cell survival and proliferation than normal group. M-WGE showed significantly higher than CMS group in cell survival. In the WGE related antidepressive pathway: Slit-robo, Slit-1 showed that chronic mild stress truly had higher level than normal and WGE treatment in hippocampus. In conclusion, WGE may had antidepressive effect though reverse neuroplasticity in chronic mild stress model.