| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 103 === During the wound healing process, the neutrophil can produce the reactive oxygen species (ROS) to kill the bacteria and prevent the infection. A nanofibrous mat with antimicrobial activity was produced by electrospinning. Glucose Oxidase (GOx) (950 U/ml) and Catalase (25 mg/ml) were incorporated in polyvinyalcohol (PVA) nanofibers by mixing enzyme into PVA solution in the volume ratio of 1:4. Both enzymes were encapsulated in the nanofibrous mat (150-250 nm fiber diameter) by simultaneous electrospinning using two separate needles at voltage 13.0-14.5 kV and 110 mm tip-to-collector distance. The activity of GOx and catalase in nanofibrous mats is 12.46±0.0310-2 U/cm2 and 67.38±4.65 U/cm2, respectively. By providing 1 ml of 5 mg/ml glucose to 0.785 cm2 nanofibrous mats, hydrogen peroxide (H2O2) will be generated first from the mat and the antibacterial activity was observed by measuring the inhibition zones of Escherichia coli and Staphylococcus aureus plates. H2O2 that produced from the GOx nanofibers can be catalyzed to generate oxygen (O2) by the catalase nanofibers of the mat. As PVA is water-soluble polymer, PVA nanofibrous mats stability required to be improved by GA cross-link and spray coating. For further application, the nanofibrous mats was stored at 4C to keep the activity of enzyme. Polyvinylpyrrolidone (PVP)-H2O2 complex was co-electrospun with PVA-Catalase nanofibers into a composite mat. without substrate, the nanofibrous mat itself can release H2O2 and show the antibacterial activity. H2O2 can be easily decomposed into water and oxygen, therefore by storing composite nanofibrous mats at -20C can maintain 95% of H2O2 amount. Two kinds of membrane prepared in this research is expected to be applied for wound dressing.
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