| Summary: | 碩士 === 國立虎尾科技大學 === 生物科技研究所 === 103 === Antrodia cinnamomea is a precious edible fungus endemic to Taiwan that has efficacy of hepatoprotective and anticancer. Numerous bioactivity studies have investigated and validation, Antrodia cinnamomea has extensive biological activities, such as anti-hepatitis B virus effects, anticancer activity et al. Traditionally, the major marker compounds of ruiting bodies of Antrodia cinnamomea were analyzed with LC-UV. Ting-Yu Lin et al. proposed a LC-UV method for 13 marker compounds which needed 120 minutes run time. In this study, the conditions of LC were improved to achieve a faster analytical method (20 min)for the profiling of the fruiting bodies of Antrodia cinnamomea. The mass spectrometric detection was also applied in this study to obtain the molecular ions and ion fragments information to reinforce the credibility in the qualitative analysis. The developed method was used to analyze various Antrodia cinnamomea related samples with or without the treatment of the Herb (Asarum). The results showed that the Asarum processed Antrodia cinnamomea still contains abundant marker components. For six months growth period samples, the lanostane-type triterpenoids were dominated ; for samples of 12 and 14 months growth, the ergostane-type triterpenoids were dominated. The composition change is consisted with the results reported by Ting-Yu Lin et al. The extracts were analyzed by different detection modes, i.e. UV, ES +, ES-, DA + and DA-. The results shown that the 13 marker compounds were all clearly detected with the LC-MS, in which 4 contents of the compounds are lese aboundent in the fruiting body. The LC-ES (+)-MS and LC-DA (+)-MS can observe the most markers (18 compounds). The signal sensitivity of LC-MS were significantly stronger than the LC-UV. Finally, we also try to develope a semi-quantitative analysis based on ursolic acid equivalent. This study has builded a fast, reliable compound profiling and semi-qutitative method for the Antrodia cinnamomea extracts by LC-UV-TOF-MS.
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