Whitening Activity of Ortho-hydroxyisoflavone in a C57BL/6J Mouse Model

• Main Authors: Jia-Xin Wu, 吳佳欣
• Other Authors: 戴守谷
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/93y9x5

碩士 === 國立虎尾科技大學 === 生物科技研究所 === 103 === The research and development of whitening agents has mostly focused on inhibiting the activity of tyrosinase in the melanogenesis pathway. Numerous whitening active ingredients currently used in clinical medicine and cosmetics are physiologically toxic, thus restricting their use. Therefore, to develop a natural and harmless whitening agents, we used a recombinant strain of Pichia pastoris harboring a cytochrome P450 fusion gene, which has the ability to produce ortho-hydroxyisoflavones. In this study, C57BL/6J mice were used for constructing an animal model to discuss the skin-whitening efficacy of the ortho-hydroxy isoflavone. The vaseline-based ointment contained 1% or 0.2% ortho-hydroxygenistein and 1% or 0.2% ortho-hydroxydaidzein to facilitate efficacy assessment. The results revealed that 0.2% genistein derivative formulation and 0.2% daidzein derivative formulation applied to the skin of mice significantly increased the average skin-whitening index (L value), indicating the potential of as a treatment for skin hyperpigmentation in humans. In the future, we expect to use this recombinant P. pastoris to produce abundant ortho-hydroxyisoflavone, which can be used to develop whitening agents with low toxicity.