| Summary: | 碩士 === 國立虎尾科技大學 === 生物科技研究所 === 103 === This designed experiments of Equisetum ramosissimum extracts were separated with ethyl acetate (EA), dichloromethane (DM), n-hexane (Hex), methanol (MeOH), and Water, respectively. Five crude extracts were screened to evaluate the antioxidant effects, containing reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging ability and Fe2+-metal chelating power evaluations. According to our result, EA extract showed the best anti-oxidative properties of five crude extracts. All of the extracts were examined the inhibitory effectiveness against human melanoma cell lines, including A375, A375.S2, A2058, and B16-F10 cells, and EA extract also presented the better anti-proliferation abilities for A375, A375.S2 and A2058 cells above 50 μg/mL. The cell viability assay in two human keratinocytes (HaCaT) and fibroblasts at dosages ranging from 0 to 100 μg/mL for 24 hours was demonstrated an acceptable outcome in normal cell safeties. The pigment melaningenesis suppression was evaluated through the melanin content assay with mouse melanoma cell, B16-F10, in vitro platform to discover skin lightening extracts. The suppressive data indicated that EA extract inhibited melanin productions, meanwhile, DM extract possessed the stimulation ability of pigment enhancements. Western blot analytics demonstrated the increase of pro-apoptotic proteins such as caspase-3 and caspase-9 after treatment with E. ramosissimum extracts. In addition, western blotting was showed that DM extract increase the melaningenesis on B16-F10 cell, the protein of the MITF, tyrosinase, TRP-1 and TRP-2 were significantly increased while the high concentration.
The totally results were showed that EA extract from E. ramosissimum has obvious effect to improve anti-oxidation, inhibit the growth of melanoma cells and reduce the melanin production.
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