Establishing a Bioassay for Testing the Estrogenic Activity in Animal Diets

• Main Authors: WANG, KAI-YING, 王凱瑩
• Other Authors: Shu-Yin Wang
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/06807886745971524568

碩士 === 中國文化大學 === 生物科技研究所 === 103 === The weather in Taiwan is hot and humid all year round, therefore is suitable for fungal growth. Maize and soybean are the main feed stuffs used in animal diets, and zearalenone, a non-steroidal estrogenic mycotoxin, if produced by Fusarium, would have an impact on growth, immunity and reproductive performance to the animals. Soybean isoflavones (ISO) is a subgroup of flavonoids found in soybean. ISO elicits antioxidant and estrogenic effects on animals. In this study, Michigan Cancer Foundation-7 (MCF-7), known to have estrogen receptor (ERα), was used to evaluate ZEA activity and the interaction of ZEA and ISO. Avian granulosa cells were also used to compare with MCF-7 for the ZEA activity by analyzing the gene expression of the steroidogenic enzymes and the StAR protein. The results showed that the growth of MCF-7 was inhibition by ZEA in a U-type non-linear doseresponse (10-8-10-3μM) manner. The interaction effect of ZEA and ISO to the MCF-7 cell exhibited the same manner. The similar cytotoxic effect was also observed in the avian granulosa cell. The progesterone (P4) secretion in avian granulosa cell was not affected by ZEA, although the gene expression of steroidogenic enzymes was slightly inhibited by ZEA, so was the ERα. It is speculated that the enzyme activities and the affinity of the ERα might have been up regulated. We have established a platform of using cell line and /or avian granulosa cells to detect the ZEA in animal diets, further study is needed to refine the system.