The Expressions of Elastin, Fibrillin-1 and Microfibril-associated Protein 4 (MFAP-4) under Oxidative Stress in Human Fibroblast Cells

• Main Authors: Peng, Li-Ling, 彭琍羚
• Other Authors: Lin, Chih-Chien
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/76295101983414283459

碩士 === 靜宜大學 === 化粧品科學系 === 103 === Skin aging is due to reduce the elastic fibers of the dermis layer, and that eventually causes skin structure loose, inelastic and wrinkled. The reasons that cause skin aging are physiological factors, for example, skin healing ability would decrease following growth. And the other reasons are environmental factors, like sunlight, and air pollution.Elastic fiber is an important component of the extracellular matrix of skin, it contributes elasticity and flexibility for skin. It consists of two major components, tropoelastin and microfibrils. One of major structural constituents of microfibrils is fibrillin-1, which is essential for the formation of elastic fibers found in connective tissue. And microfibril-associated protein 4 (MFAP-4) is an important protein to help fibrillin-1 to cross link and tight the structure. In recent years, elastic fiber would be synthesized aberrantly and it would be more wrinkle in the appearance if human are exposure in long time. Therefore, in this study, we try to use oxidants to produce reactive oxygen species (ROS) to simulate a oxidative stress environment to culture Detroit 551 human fibroblast cells, and then analyze the expressions of tropoelastin, fibrillin-1, and microfibril-associated protein 4 in Detroit 551 human fibroblast cells. According to experiment result, hydrogen peroxide can reduce the fibrillin-1 mRNA expression, but increase MFAP 4 mRNA expression in Detroit 551 cells. Sodium nitrite doesn’t influence the fibrillin-1 and MFAP 4 mRNA expression in Detroit 551 cells. Two oxidants both reduce wound healing ability in Detroit 551 human fibroblast cells, and the structural proteins are aberrant.