Development of artificial tear fluid contained anti-inflammation &; anti-oxidation agents for dry eye syndrome treatment

• Main Authors: Hung Chang Chen, 陳泓璋
• Other Authors: 方旭偉
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/6fxshk

碩士 === 國立臺北科技大學 === 化學工程研究所 === 103 === Dry eye syndrome (DES) is one of the most common ocular disease in the world. In this study, it is going to discuss how to treat dry eye syndrome more efficiently. Artificial tear is the most common eye-drop to treat DES, but usually with preservatives supplement, the addition of preservatives could resulted in a sever condition of DES. make dry eye phonama more deteriorate. DES usually accompany with ocular inflammation. To treat the inflammation on the ocular tissue is the strategy. Kempferol (KM) with anti-inflammatory effect was selected to added in the artificial tear for inflammation inhibition. an anti-oxidation agent, Ferulic acid (FA) was used to replace the preservatives. . In this study, an artificial tear fluid contain anti-flammation agent (Kaempferol, KM) and anti-oxidation agent (Ferulic Acid, FA) for DES treatment was prepared and evaluated. The cell viability, cytotoxicity and anti-inflammaion effect were examined in vitro by co-cultured with human cornea epithelial cell(HCE), to find out the optimal concentration of KM and FA to treat HCE cell. Then a DES rabbit model was established, and the artificial tear solution with KM(1 μM) + FA(100 μM) as eye drop was used, tear production, cornea damage, and ocular irritation were examined for evaluate the therapeutic effect.