| Summary: | 碩士 === 國立臺北科技大學 === 化學工程與生物科技系生化與生醫工程碩士班 === 103 === We have designed and synthesized a new long-wavelength fluorescent probe BBCH for the sensitive determination of physiologically important thiols, cysteine and homocysteine. The fluorogenic chemical transformation of BBCH triggered by thiols is a spontaneous and irreversible process at physiological temperature. The fluorescence probe BBCH is non-fluorescent. In the presence of either cysteine or homocysteine, BBCH undergoes a SN2 reaction with concomitant release of long wavelength fluorogenic BCH along with the formation 5-oxothiomorpholine-3-carboxylic acid as byproduct. The fluorescence changes were linearly dependent with the concentration of cysteine and homocysteine. To detect cysteine, the described fluorescence sensor presents linear range of 1μM to 10μM and detection limit of 0.87μM. To detect homocysteine, the described fluorescence sensor presents linear range of 1μM to 10μM and detection limit of 1.90μM. The fluorescence signal of BBCH is rapid and specific to cysteine. The probe exhibits excellent selectivity to detect cysteine over 19 other amino acids. Moreover, practical applicability of the described sensor has been assessed in biological and pharmaceutical samples.
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