A study on the separation and purification of hyaluronic acid by using polyvinylidene fluoride membrane

• Main Authors: Yen-Ju Wu, 吳彥儒
• Other Authors: Cheng-Tung Wen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/63991777608045564088

碩士 === 淡江大學 === 化學工程與材料工程學系碩士班 === 103 === The synthesized polyvinylidene fluoride (PVDF) flat-sheet membrane was used in concentrate filtration and diafiltration for separation and purification of hyaluronic acid. The flat membranes were prepared from the dope solution of PVDF, Triethyl phosphate(TEP) and Tween-20, and varying the concentration of TEP in the coagulation bath. The membrane structures were observed by the SEM analysis. In this study, the HA solution and the mixture solution of HA/LY were used as the feed solution, and experimented by adopting Concentrate filtration and diafiltration in a dead-end stirred cell. The experimented results were discussed under different operating conditions such as stirred rate, electrolyte type and electrolyte concentration. The results shows that concentrate filtration’s concentration and accumulation filtrate better than original membranes which prepared by higher weigh percent of TEP solution. In the diafiltration for HA the flux higher performance than original membranes. Membrane have large pore size in the top surface is increasing the weight percent of TEP in coagulant bath. Addition of stirring can reduce the filtration resistance and improve the permeate flux. The permeate flux decreases with the increase of ion strength of electrolyte which causes the HA molecules become more shrunken and then form a compact deposited layer on membrane surface. However, the LY molecules can pass through the membrane more easily as the electrolyte is added. The strength of electrode ion will hinder the surface charge of LY, and decrease the electrostatic interference between LY molecules and membrane.