| Summary: | 碩士 === 國立陽明大學 === 生命科學系暨基因體科學研究所 === 103 === Neovascularization plays a role in several pathological conditions, including tumor growth, arthritis, and choroidal neovascularization. Preliminary in vitro Matrigel angiogenesis assays on the EtOH extracts of Chinese herbal medicine revealed that the hexane layer of Lespedeza cuneata was active against the formation of tube-like structures on Matrigel. Lespedeza cuneata is a perennial legume distributed in Korea, China, India, Australia and Taiwan and used as folk anti-inflammatory medicine. By anti-angiogentic activity-guided silica gel chromatography of the hexane layer, a low-polarity fraction containing polyunsaturated fatty acids with IC50 of 8 μg/ml was obtained. It had been proved that long chain n-3 polyunsaturated fatty acids have anti-angiogenic effect in intestinal microvascular endothelium. We also isolated and identified 3 triterpenes (LC-3 &; 4) among which stigmasterol and β-sitosterol had been reported to have anti-inflammatory properties along with two other compounds, salicylic acid (LC-1) and (6S,7E)-vomifoliol (LC-2).
Gaillardia pulchella is a annual asteraceae native to the Central United States and filled with sesquiterpene lactones which in recent studies has been shown to be associated with anti-tumor, cytotoxic, anti-microbial and phytotoxic activity. And there is the chemotaxonomic variation in their chemical constituents depending on locale of cultivation. Four pseudoguaianolides (GP-1~5) were isolated from the EtOAc layer of G. pulchella ethanolic extract together with one flavonoid, eupafolin (GP-6). Eupafolin was for the first time isolated from this plant.
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