Effects of HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5H)-furanone] on U87-MG Human Glioblastoma Cells
碩士 === 國立陽明大學 === 解剖學及細胞生物學研究所 === 103 === Compound HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2 (5H)-furanone] containing a cis-stilbene moiety is a new analogue of the antitumor compound combretastatin A-4 (CA-4). Glioblastoma multiforme (GBM) is the most aggressive malignant primary tumo...
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ndltd-TW-103YM0053910022016-08-22T04:17:15Z http://ndltd.ncl.edu.tw/handle/55684924698197631927 Effects of HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5H)-furanone] on U87-MG Human Glioblastoma Cells HYS-32 對於人類多形性神經膠母細胞瘤 U87-MG 之探討 Zong-Han Tsai 蔡宗翰 碩士 國立陽明大學 解剖學及細胞生物學研究所 103 Compound HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2 (5H)-furanone] containing a cis-stilbene moiety is a new analogue of the antitumor compound combretastatin A-4 (CA-4). Glioblastoma multiforme (GBM) is the most aggressive malignant primary tumor in human brains. The effects of HYS-32 on GBM remain unknown. In this study, we investigated the effects of HYS-32 in the U87-MG human glioblastoma cell line and the signaling pathway involved. MTT assay demonstrated that HYS-32 causes a cytotoxic effect in U87-MG cells in a dose-dependent manner. Apo-Brdu-IHC in situ DNA fragmentation analysis and flow cytometry indicated cellular apoptosis and cell cycle arrest in U87-MG cells. Immunoblot analyses showed that HYS-32 induces a dose- and time-dependent increase in cytochrome c and cleaved caspase-3 protein levels, indicating cellular apoptosis in U87-MG cells. Moreover, immunoblot analyses also showed that HYS-32 induces a dose- and time-dependent increase in Beclin 1 and LC3-II protein levels but decrease in SQSTM1 levels, suggesting cellular autophagy in U87-MG cells. HYS-32 also induced aggregation of LC3 protein in cytoplasm, disassembly of microtubules, and morphology changes in U87-MG cells. Co-treatment of HYS-32 and Beclin 1 inhibitor Spautin 1 prevented the HYS-32-induced Beclin 1 upregulation and autophagy. Taken together, our results demonstrated that HYS-32 induces microtubule disassembly, cell cycle arrest, cellular apoptosis, and autophagy in U87-MG cells. The novel biological effects of HYS-32 on glioblastoma may become a new potential as a therapeutic drug used for treatment of human glioblastoma. Jiahn-Chun Wu 吳建春 2015 學位論文 ; thesis 53 en_US |
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碩士 === 國立陽明大學 === 解剖學及細胞生物學研究所 === 103 === Compound HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2
(5H)-furanone] containing a cis-stilbene moiety is a new analogue of the antitumor compound combretastatin A-4 (CA-4). Glioblastoma multiforme (GBM) is the most aggressive malignant primary tumor in human brains. The effects of HYS-32 on GBM remain unknown. In this study, we investigated the effects of HYS-32 in the U87-MG human glioblastoma cell line and the signaling pathway involved. MTT assay demonstrated that HYS-32 causes a cytotoxic effect in U87-MG cells in a dose-dependent manner. Apo-Brdu-IHC in situ DNA fragmentation analysis and flow cytometry indicated cellular apoptosis and cell cycle arrest in U87-MG cells. Immunoblot analyses showed that HYS-32 induces a dose- and time-dependent increase in cytochrome c and cleaved caspase-3 protein levels, indicating cellular apoptosis in U87-MG cells. Moreover, immunoblot analyses also showed that HYS-32 induces a dose- and time-dependent increase in Beclin 1 and LC3-II protein levels but decrease in SQSTM1 levels, suggesting cellular autophagy in U87-MG cells. HYS-32 also induced aggregation of LC3 protein in cytoplasm, disassembly of microtubules, and morphology changes in U87-MG cells. Co-treatment of HYS-32 and Beclin 1 inhibitor Spautin 1 prevented the HYS-32-induced Beclin 1 upregulation and autophagy. Taken together, our results demonstrated that HYS-32 induces microtubule disassembly, cell cycle arrest, cellular apoptosis, and autophagy in U87-MG cells. The novel biological effects of HYS-32 on glioblastoma may become a new potential as a therapeutic drug used for treatment of human glioblastoma.
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author2 |
Jiahn-Chun Wu |
author_facet |
Jiahn-Chun Wu Zong-Han Tsai 蔡宗翰 |
author |
Zong-Han Tsai 蔡宗翰 |
spellingShingle |
Zong-Han Tsai 蔡宗翰 Effects of HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5H)-furanone] on U87-MG Human Glioblastoma Cells |
author_sort |
Zong-Han Tsai |
title |
Effects of HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5H)-furanone] on U87-MG Human Glioblastoma Cells |
title_short |
Effects of HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5H)-furanone] on U87-MG Human Glioblastoma Cells |
title_full |
Effects of HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5H)-furanone] on U87-MG Human Glioblastoma Cells |
title_fullStr |
Effects of HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5H)-furanone] on U87-MG Human Glioblastoma Cells |
title_full_unstemmed |
Effects of HYS-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5H)-furanone] on U87-MG Human Glioblastoma Cells |
title_sort |
effects of hys-32 [4-(3,4-dimethoxyphenyl)-3-(naphthalen-2-yl)-2(5h)-furanone] on u87-mg human glioblastoma cells |
publishDate |
2015 |
url |
http://ndltd.ncl.edu.tw/handle/55684924698197631927 |
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