| Summary: | 碩士 === 國立陽明大學 === 環境與職業衛生研究所 === 103 === Background: Attempts to identify biomarkers predictive of the development of atopic dermatitis (AD) have been made by many investigators. However, urine biomarkers were not fully studied.
Objective: To investigate whether urine 8-OHdG can serve as a biomarker for identifying children with risk of AD.
Method: A nested case-control study with patients selected from population-based cohort of childhoo d-onset atopic dermatitis was performed. Urinary analysis and urine 8-OHdG were checked for 200 children with AD and 200 controls selected from Childhood Environment and Allergic diseases Study (CEAS) cohort. Information about allergic disease prevalence and environmental exposures was collected by employing International Study of Asthma and Allergies in Childhood (ISAAC) questionnaire. Multiple logistic regression analyses were performed to estimate the association between urine 8-OHDG and the risk of AD with adjustment for potential confounders.
Result: The urinary 8-OHdG concentrations in AD children were significantly higher than those in the controls (mean±SD 213.68±166.07 v.s.157.33±105.40 ng/mg Cr; p<0.001). The risk of AD was found to increase with urine 8-OHdG levels in a dose-response manner (p for trend= 0.02). High urine 8-OHdG levels (>75th percentile) were significantly associated with AD with an OR of 2.87 (95% CI 1.61-5.10) after adjusting for potential confounders. The frequencies of night pruritus were positively correlated with 8-OHdG levels (r= 0.17, p= 0.01). AD children who had high urine 8-OHdG level were more likely to develop asthma with an OR of 2.70 (95% CI 1.11-6.56).
Conclusion: Urinary 8-OHdG may serve as a biomarker for evaluating the risk of process of “atopic march” in children with AD. It is also correlated with night pruritus. Oxidative stress may play an important role in atopic disorders.
Key words: Oxidative stress, atopic dermatitis, atopic disorders, asthma, allergic rhinitis, atopic march, 8-OHdG, urine biomarker
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