| Summary: | 碩士 === 長庚大學 === 生物醫學研究所 === 104 === Cholangiocarcinoma (CC), the second most common primary
malignant hepatic tumor that arises from biliary epithelial cells, is recently
increasing in incidence and has poor prognosis. Currently, only surgical
resection and liver transplantation can completely cure CC in early-stage,
but it has no specific symptoms at early-stage and difficult to diagnose
owing to low specificity of most diagnostic modalities and biomarkers to
differentiate with other hepatobiliary diseases. The lack of a sensitive and
specific diagnostic marker and alternative treatments are the main reasons
why patients with CC have a very poor outcome. Mass spectrometry-based
proteomics is high throughput, sensitive and accurate, representing a
powerful approach for the identification of biomarkers with potential
clinical utility. Bile could be extremely valuable to discover biomarkers of
CC, because it is directly secreted by the biliary tract. In this thesis, we
identified 152 differentially expressed proteins in bile of the benign biliary
disease – gallstones patients (n=2) and CC patients (n=4) through twodimensional
liquid chromatography tandem mass spectrometry. In addition,
we established a CC biomarker database in three ways : (1) Search in
PubMed for 115 reported protein biomarkers ； (2) Search for 623
differentially expressed genes from microarray datasets in Gene Expression
Omnibus(GEO) database；(3) Search for secretome and exosome database.
The combination of these three CC biomarker-related datasets and our data
for the identifying differentially expressed proteins in bile should greatly
facilitate the development of highly sensitive and specific protein
biomarker of CC in the future.
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