| Summary: | 碩士 === 長庚大學 === 機械工程學系 === 104 === Endovascular repair for mycotic aortic aneurysm is a less invasive alternative to open surgery, although placement of a stent-graft in an infected environment remains controversial. In this study, we developed hybrid biodegradable vancomycin-eluting nanofiber-loaded endovascular prostheses and evaluated the release of antibiotics both in vitro and in vivo. Antibiotic-eluting nanofibers were fabricated by first dissolving poly(D,L)-lactide-co-glycolide and vancomycin in 1,1,1,3,3,3- hexafluoro-2-propanol, which was then electrospun into nanofibrous tubes. The tubes were then mounted onto commercially available stent-grafts and bare metal stents. The in vitro release rates of the pharmaceuticals from the nanofibers are characterized using a 3-D printed artificial aorta and a circulation model. The experimental results suggested that the biodegradable nanofibers released high concentrations of vancomycin (well above the MIC90) for 30 days. The in vivo delivery of vancomycin was examined by implanting nanofibers loaded metal stents in rabbit aortas. The measured results suggested that the nanofibers released high concentrations of vancomycin for 8 weeks in vivo. The proposed biodegradable vancomycin-eluting nanofibers/stent grafts significantly contribute to achieve local and sustainable delivery of anti-microbial agents at infected arterial walls, and may provide potential applications in the treatment of infected aortic aneurysm.
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