| Summary: | 碩士 === 中山醫學大學 === 醫學研究所 === 104 === ZAK belongs to the mixed lineage kinase (MLK) subfamily which is a member of MAP kinase kinase kinase ( MAP3K ) family, and consists of 800 amino acids, the molecular weight is 91 KDa. Previous studies have shown that overexpression of ZAK induced apoptosis in hepatoma cells, increased cell proliferation and transformation in skin cancer cells, induced hypertrophic growth in cardiomyoblast cells, and promoted cell migration in colorectal cancer cells. In lung cancer, overexpression of ZAK will decrease cell proliferation, migration, colony formation and tumor size. ZAK comprises three functional domains, kinase catalytic domain (KD), leucine zipper (LZ) and sterile α motif (SAM). To explore the role of different ZAK fragments in proliferation and metastasis of lung cancer cells, we performed cell counting, MTT assay, colony formation, wound healing and migration assay in H460 and H1299 lung cancer cell lines. These results showed that all of the different ZAK fragments could inhibit cell growth and migration ability. The similar inhibition results were obtained when deleted both LZ and SAM domains, especially in H1299 cell line. Taken together, we thought KD regulates cell proliferation and migration, LZ is correlated with migration, SAM doesn’t affect cell proliferation and migration ability.
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