| Summary: | 碩士 === 中原大學 === 化學工程研究所 === 105 === In modern day chemical engineering processes, enzyme based catalysis is considered highly specific and environmental-friendly option. Among industrial enzymes, lipase are widely used in hydrolysis, esterification and transesterification processes. Combination of immobilization enzyme technology and membrane fabrication technology to increase enzyme efficiency had been a research focus for simultaneous catalysis and separation.
In this work, lipase from porcine pancreas and Candida rugose were immobilized onto chitosan/graphene oxide composite membrane to verify this novel material for membrane enzyme bioreactor. Since the graphene oxide have superior mechanical properties and active functional groups for enzymes to interact, it was first hypothesized that the composite membrane would show improved operational characteristics over traditional chitosan based immobilization strategy. To immobilized the enzymes onto these membranes, 1-ethyl-(3-dimethylaminopropyl) carbodiimide(EDC) and N-hydroxyl succinimide (NHS) coupling agents were applied and compared to achieve optimized result for lipase activity after immobilization. FTIR and XPS were used to analyze the surface after modification.
Besides, ratios of added GO would influence the membranes swelling ratio and parameters regarding mechanical strength of membranes were also investigated. Furthermore, the mass transfer characteristics of the composite membranes was determined through pervaporation experiments. Eventually, morphologies of the CS/GO composite membrane were characterized using the FESEM before and after prolonged operation to confirm the enhanced membrane integrity. Through combining the advantages of both the membrane technique and biocatalyst, we expected that this composite membrane could be applied in modular enzymatic membrane bioreactors on hydrolysis, esterification and transesterification reaction.
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