| Summary: | 碩士 === 中原大學 === 生物科技研究所 === 104 === Berberine is an alkaloid, which can be isolated from a lot of herb materials, with a long history of medicinal utilization in the traditional Chinese medicine. Currently, the effects of berberine have been confirmed in several pharmacological researches, including prevention of bacterial diarrhea, inhibition of cardiac dysrhythmia, inhibition of vascular smooth muscle proliferation, decrease of cholesterol level, and promotion of healing in people with diabetes.
Our previous in vitro experiments have already confirmed that berberine can act as an apoptosis inducer on late-stage embryo. However, the effect of berberine on early-stage embryo remains unclear. To investigate the effects of berberine on early-stage embryos, both of in vitro and in vivo experiments were designed. We found that failed growing of two-cell stage embryo shows a positive correlation to the concentration of bererine, in which experiment was carried out by in vitro growing two-cell stage embryo with the treatment of berberine. In vivo experiment was performed by the intra-peritoneal administration of various dosage berberine. The results from in vivo experiment showed that the increasing of berberine concentrations resulted in the significant decrease of embryo numbers, but the difference in early-stage embryo development was not significant. These study results show that berberine has harmful effect on the mouse early-stage embryo development.
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