Fabrication of Microfluidic Blood Typing Chip and Its Optical Assay Analysis

• Main Authors: Hong-Wei You, 游宏偉
• Other Authors: Yaw-Jen Chang
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/58157142442207425122

碩士 === 中原大學 === 機械工程研究所 === 104 === This study presents a novel microfluidic blood typing chip. The blood sample and reagents are driven by the capillary force, surface tension and gravity, without requiring any apparatuses for fluid manipulation. Chip fabrication was based on the photolithography process to produce a mold, followed by casting PDMS into the mold. Finally, the PDMD layer was bonded with a glass substrate. For blood typing measurement, an oscillator was used to reduce the precipitation time of blood agglutination. At the bottom of reaction vessel, a V-shape was designed. In addition, a vent connecting to the V-shaped vessel was used to release gas and prevent the occurrence of bubbles. Bioassay was based on the test protocol of manual polybrene (MP) technique. However, we used diluted whole blood in our experiments instead of centrifuged 3%-diluted red blood cells (RBC), which is used in the MP method. After the assay, the light intensity was detected to determine the result of blood typing.The experimental results show in this proposed chip the sample and reagents can be manipulated for the blood reaction without extra forces. The blood agglutination can be effectively precipitated. The diluted whole blood gives the same assay result as the centrifuged 3%-diluted RBC. We also concluded the optimal position for measurement in this proposed chip.