To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia
碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 104 === Chronic myeloid leukemia (CML) is a disease of hematopoietic stem cells arising from spontaneous chromosomal translocation. Epidemiology studies showed a slight male preponderance and women has a survival advantage over men. The mechanism of spontaneous chromos...
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ndltd-TW-104KMC055340332019-05-15T23:09:07Z http://ndltd.ncl.edu.tw/handle/xhq5gr To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia 探討SPZ 1 在慢性骨髓性白血病中癌幹細胞特性之研究 Kean-Yee, Lai 賴健瑜 碩士 高雄醫學大學 醫學研究所碩士班 104 Chronic myeloid leukemia (CML) is a disease of hematopoietic stem cells arising from spontaneous chromosomal translocation. Epidemiology studies showed a slight male preponderance and women has a survival advantage over men. The mechanism of spontaneous chromosomal translocation is still unknown. Basic helix-loop helix transcription factor (bHLH) has a key role during embryogenesis, regardless cell proliferation, cell growth and differentiation. Spermatogenesis specific leucine zipper 1 (SPZ 1) is one of the bHLH family members. It’s also known as proto-oncogene. The objective of the present thesis was to explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in CML. K562 is a CML cell line. By conducting lentivirus vector transduction method, SPZ 1 cDNA was overexpressed in K562 cell line. An increased of cell growth was observed. However, when knocked down SPZ 1 gene (SPZ 1 sh-RNAi) was transfected into K562, cell growth was restricted. Thus, in this study, the cancer stem cells’ properties were observed by comparing K562 empty vector cells, K562-Spz1 overexpressed cells, and K562-shSPZ1 cells. Western blot analysis, real-time-PCR, Fluorescence-activated cell sorting (FACS) were done. Results showed that enhanced Spz1 expression leads to cell growth acceleration whereas knocked down of Spz1 inhibited cell growth. Furthermore, enhanced Spz1 also increased factors associated with stem cell properties. Thus, these results concluded that Spz1 acted as modulator of cancer stem cells properties in CML. Shih-Hsien Hsu 許世賢 2016 學位論文 ; thesis 81 en_US |
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碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 104 === Chronic myeloid leukemia (CML) is a disease of hematopoietic stem cells arising from spontaneous chromosomal translocation. Epidemiology studies showed a slight male preponderance and women has a survival advantage over men. The mechanism of spontaneous chromosomal translocation is still unknown.
Basic helix-loop helix transcription factor (bHLH) has a key role during embryogenesis, regardless cell proliferation, cell growth and differentiation. Spermatogenesis specific leucine zipper 1 (SPZ 1) is one of the bHLH family members. It’s also known as proto-oncogene. The objective of the present thesis was to explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in CML. K562 is a CML cell line. By conducting lentivirus vector transduction method, SPZ 1 cDNA was overexpressed in K562 cell line. An increased of cell growth was observed. However, when knocked down SPZ 1 gene (SPZ 1 sh-RNAi) was transfected into K562, cell growth was restricted. Thus, in this study, the cancer stem cells’ properties were observed by comparing K562 empty vector cells, K562-Spz1 overexpressed cells, and K562-shSPZ1 cells. Western blot analysis, real-time-PCR, Fluorescence-activated cell sorting (FACS) were done. Results showed that enhanced Spz1 expression leads to cell growth acceleration whereas knocked down of Spz1 inhibited cell growth. Furthermore, enhanced Spz1 also increased factors associated with stem cell properties. Thus, these results concluded that Spz1 acted as modulator of cancer stem cells properties in CML.
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Shih-Hsien Hsu |
author_facet |
Shih-Hsien Hsu Kean-Yee, Lai 賴健瑜 |
author |
Kean-Yee, Lai 賴健瑜 |
spellingShingle |
Kean-Yee, Lai 賴健瑜 To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia |
author_sort |
Kean-Yee, Lai |
title |
To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia |
title_short |
To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia |
title_full |
To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia |
title_fullStr |
To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia |
title_full_unstemmed |
To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia |
title_sort |
to explore the potential regulatory effect of spz 1 on the cancer stem cells’ properties in chronic myeloid leukemia |
publishDate |
2016 |
url |
http://ndltd.ncl.edu.tw/handle/xhq5gr |
work_keys_str_mv |
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