To explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in chronic myeloid leukemia

• Main Authors: Kean-Yee, Lai, 賴健瑜
• Other Authors: Shih-Hsien Hsu
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/xhq5gr

碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 104 === Chronic myeloid leukemia (CML) is a disease of hematopoietic stem cells arising from spontaneous chromosomal translocation. Epidemiology studies showed a slight male preponderance and women has a survival advantage over men. The mechanism of spontaneous chromosomal translocation is still unknown. Basic helix-loop helix transcription factor (bHLH) has a key role during embryogenesis, regardless cell proliferation, cell growth and differentiation. Spermatogenesis specific leucine zipper 1 (SPZ 1) is one of the bHLH family members. It’s also known as proto-oncogene. The objective of the present thesis was to explore the potential regulatory effect of SPZ 1 on the cancer stem cells’ properties in CML. K562 is a CML cell line. By conducting lentivirus vector transduction method, SPZ 1 cDNA was overexpressed in K562 cell line. An increased of cell growth was observed. However, when knocked down SPZ 1 gene (SPZ 1 sh-RNAi) was transfected into K562, cell growth was restricted. Thus, in this study, the cancer stem cells’ properties were observed by comparing K562 empty vector cells, K562-Spz1 overexpressed cells, and K562-shSPZ1 cells. Western blot analysis, real-time-PCR, Fluorescence-activated cell sorting (FACS) were done. Results showed that enhanced Spz1 expression leads to cell growth acceleration whereas knocked down of Spz1 inhibited cell growth. Furthermore, enhanced Spz1 also increased factors associated with stem cell properties. Thus, these results concluded that Spz1 acted as modulator of cancer stem cells properties in CML.