Preparation of Polylactic Acid/Rice Husk Ash Particles Mixed Matrix Membranes for Adsorption of Lysozyme from Chicken Egg White

• Main Authors: Chih-Yi Cheng, 鄭芷懿
• Other Authors: 孫幸宜
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/86211722455974912555

碩士 === 國立中興大學 === 化學工程學系所 === 104 === This study focuses on the preparation of porous mixed matrix membrane (MMM) by filling rice husk ashes (RHA) particles in polylatic acid (PLA), specifically for adsorptive lysozyme from chicken egg white. RHA was adopted as fillers to fabricate the MMM via water-vapor-induced phase inversion. The RHA and MMM were characterized by BET, FTIR, SEM, TEM, TGA, zeta potential, and ion-exchange capacity (IEC). The adsorption capacity was 1006 μmol/g for RHA and 844 μmol/g for MMM. In batch process, the protein adsorption equilibrium was fully established within 2 hours of contact time. The optimal pH for a better separation of lysozyme would be at 8. The adsorptivity of the MMM was essentially contributed from the filled rice husk ashes particles. After a batch adsorption-washing-elution cycle, the lysozyme adsorption about 74%, recovery about 73% with the purity near 100% by using RHA. In the flow process (dead-end) with a piece of 25 mm MMM disc for 0.5 mg/mL lysozyme solution, the optimal flow rate was 1 mL/min and the number of bed volume was 49.4. After an adsorption-washing-elution cycle to purify lysozyme from chicken egg white, the lysozyme adsorption about 72%, recovery about 71% with the purity near 100% by using MMM. The MMM performance could be effectively repeated in the dead-end operation.