| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 104 === Dendritic cells (DCs) link innate immune sensing of the environment to the initiation of adaptive immune responses. Accordingly, DCs are considered as a major target in the development of immunomodulators. Menthol is a naturally monoterpene isolated from the essential oil of peppermint or other mint plants. While substantial evidence indicates that menthol possesses immunomodulatory activity, however, the effect of menthol on dendritic cells (DCs) remains unknown. In this study, we investigated the effect of menthol on lipopolysacchride (LPS) stimulated mouse bone marrow-derived DCs activation. Our experimental results show that menthol could reduce the pro-inflammatory cytokines (TNF-α, IL-6), chemokine (IL-8, MCP-1) and Th1 cytokine IL-12 expression on LPS-activated DC and this reduction, however, it did not affect the expression of of MHC or costimulatory (CD11c、CD40、CD80、CD86) molecules and did not influence the ability to take up antigen. Furthermore, menthol also attenuates the Th1-type immune response in 2,4-Dinitro-1-fluorobenzene pulsed DC-transfer model of contact hypersensitive (CHS) in vivo. Collectively, our findings suggest that menthol can manipulate the inflammatory properties of DCs and furthermore, impedes the function of DCs to prime a Th1-mediated immune response. These results provide new insight into the immunopharmacological role of menthol and applied for treatment of chronic inflammatory disorders or Th1-mediated autoimmune diseases.
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