Immune Analysis of HbA1c by Micro-Mass Sensing Chips

• Main Authors: Siou-WunYe, 葉修文
• Other Authors: Yu-Cheng Lin
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/98742046222907274067

碩士 === 國立成功大學 === 工程科學系 === 104 === This study presents a method to detect the concentration of glycated hemoglobin (HbA1c) and hemoglobin (Hb) by frequency variation value measurement. This experiment is based on the immunological principle, so we first found out the best antibody concentration that is coated on the chips to catch the antigens we want. In this experiment, cystamine medicines for electrode reaction zone can be chemically modified by the immune reaction time test, glycated hemoglobin and hemoglobin of reaction time are bonded to complete within seven minutes. Experimental results, glycated hemoglobin detection range of 1~5000 ng/mL and limit concentration is 5300 ng/mL, hemoglobin detection range of 10~20000 ng/mL and limit concentration is 22000 ng/mL. Finally, we successful detect the mix sample of glycated hemoglobin and hemoglobin with 2~16% (ratio of glycated hemoglobin in total hemoglobin).