Functional Polymeric Mixed Micelles Based on Amphiphilic Copolymers: Preparation, Characterization, and Application in Drug Carriers

• Main Authors: Hsing-ChunChen, 陳興群
• Other Authors: Wen-Chung Wu
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/8ksxjd

碩士 === 國立成功大學 === 化學工程學系 === 104 === In this study, we prepare a mixed micelle system as drug carriers. The mixed micelles were co-assembled of three amphiphilic copolymers, [PCL-b-P(TEGMA-co-FA)], [PCL-b-P(TEGMA-co-AHA)] and [PCL-b-P(TEGMA-co-(PPS-HEMA))]. PCL block is hydrophobic and used as the core of micelle to encapsulate hydrophobic drug, DOX. PTEGMA block is thermo-sensitive and acts as the hydrophilic shell of micelle. In addition, we introduced the pH-sensitive, active targeting and fluorescent monomer (AHA, FA and PPS-HEMA) to the hydrophilic block by copolymerization the corresponding comonomers with TEGMA, respectively. The nanostructure, stimuli-responsive properties, active targeting properties, florescence behaviors and cytotoxicity for tumor cells were investigated. With delicate control of the composition of AHA in the hydrophilic block, the LCST of mixed micelles exhibited pH-dependent thermal transition behaviors suitable for drug delivery systems that LCST is higher than body temperature (37℃) at neutral environment but lower than body temperature at acidic environment. Therefore, the drug can be encapsulated in the core of micelle stably in blood circulation and released in tumor which is acidic because the micellar structure is destroyed. FA can conjugate with the targeted tumor with FA receptors overexpressed on the surface to make drug accumulate more efficiently. PPS-HEMA is a florescence monomer with AIE effect, and FRET will occur when the spectral overlap and close proximity between PPS-HEMA and DOX. Based on FRET effect, we are able to analyze if DOX was successfully encapsulated in the mixed micelle.