Regulation of enterovirus A71 infection by tumor suppressor WW domain-containing oxidoreductase
碩士 === 國立成功大學 === 醫學檢驗生物技術學系 === 104 === Human fragile WWOX gene encodes a tumor suppressor WW domain-containing oxidoreductase (designated WWOX). WWOX protein has been shown to regulate embryonic development, bone metabolism, neural injury and apoptosis through various signaling pathways. Previou...
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ndltd-TW-104NCKU51081142017-10-01T04:30:11Z http://ndltd.ncl.edu.tw/handle/06946807235120773817 Regulation of enterovirus A71 infection by tumor suppressor WW domain-containing oxidoreductase 腫瘤抑制蛋白質含雙色胺酸功能區氧化還原酶對於腸病毒A71型感染症的調控 Pei-ShiuanChen 陳佩璇 碩士 國立成功大學 醫學檢驗生物技術學系 104 Human fragile WWOX gene encodes a tumor suppressor WW domain-containing oxidoreductase (designated WWOX). WWOX protein has been shown to regulate embryonic development, bone metabolism, neural injury and apoptosis through various signaling pathways. Previous studies have suggested that WWOX interacts with EB virus latent membrane protein 2A and HTLV-1 oncoprotein Tax, thus leading to tumorigenesis. However, whether WWOX affects viral infection remains unknown. Enterovirus A71 (EV-A71) belongs to the Enterovirus genus within the family of Picornaviridae. In this study, we found that Wwox-/- mice showed higher mortality rates and clinical scores as compared with Wwox+/+ and Wwox+/- littermates after intraperitoneal or oral infection of EV-A71. Higher viral loads were detected in multiple organs of EV-A71-infected Wwox-/- mice. These results suggest that WWOX may downregulate EV-A71 infection in vivo. To investigate how host factor WWOX downregulates EV-A71 infection, we examined EV-A71 life cycle in WWOX-knockdown cell lines. In summary, our results suggest that WWOX negatively regulates EV-A71 infection both in vivo and in vitro. In the future, WWOX-mediated signaling pathways that regulate EV-A71 infection will be further investigated. Li-Jin Hsu 徐麗君 2016 學位論文 ; thesis 84 en_US |
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碩士 === 國立成功大學 === 醫學檢驗生物技術學系 === 104 === Human fragile WWOX gene encodes a tumor suppressor WW domain-containing oxidoreductase (designated WWOX). WWOX protein has been shown to regulate embryonic development, bone metabolism, neural injury and apoptosis through various signaling pathways. Previous studies have suggested that WWOX interacts with EB virus latent membrane protein 2A and HTLV-1 oncoprotein Tax, thus leading to tumorigenesis. However, whether WWOX affects viral infection remains unknown. Enterovirus A71 (EV-A71) belongs to the Enterovirus genus within the family of Picornaviridae. In this study, we found that Wwox-/- mice showed higher mortality rates and clinical scores as compared with Wwox+/+ and Wwox+/- littermates after intraperitoneal or oral infection of EV-A71. Higher viral loads were detected in multiple organs of EV-A71-infected Wwox-/- mice. These results suggest that WWOX may downregulate EV-A71 infection in vivo. To investigate how host factor WWOX downregulates EV-A71 infection, we examined EV-A71 life cycle in WWOX-knockdown cell lines. In summary, our results suggest that WWOX negatively regulates EV-A71 infection both in vivo and in vitro. In the future, WWOX-mediated signaling pathways that regulate EV-A71 infection will be further investigated.
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author2 |
Li-Jin Hsu |
author_facet |
Li-Jin Hsu Pei-ShiuanChen 陳佩璇 |
author |
Pei-ShiuanChen 陳佩璇 |
spellingShingle |
Pei-ShiuanChen 陳佩璇 Regulation of enterovirus A71 infection by tumor suppressor WW domain-containing oxidoreductase |
author_sort |
Pei-ShiuanChen |
title |
Regulation of enterovirus A71 infection by tumor suppressor WW domain-containing oxidoreductase |
title_short |
Regulation of enterovirus A71 infection by tumor suppressor WW domain-containing oxidoreductase |
title_full |
Regulation of enterovirus A71 infection by tumor suppressor WW domain-containing oxidoreductase |
title_fullStr |
Regulation of enterovirus A71 infection by tumor suppressor WW domain-containing oxidoreductase |
title_full_unstemmed |
Regulation of enterovirus A71 infection by tumor suppressor WW domain-containing oxidoreductase |
title_sort |
regulation of enterovirus a71 infection by tumor suppressor ww domain-containing oxidoreductase |
publishDate |
2016 |
url |
http://ndltd.ncl.edu.tw/handle/06946807235120773817 |
work_keys_str_mv |
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