Construction of database of translational regulation motifs in mRNA 5'UTR to explore the control mechanism of protein synthesis

• Main Authors: Yu-ChengWang, 王語琤
• Other Authors: Wei-Sheng Wu
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/97342037216415754256

碩士 === 國立成功大學 === 電機工程學系 === 104 === The translational regulation mechanisms of gene expression can be divided into two types: Cap-dependent and IRES-dependent. (Internal Ribosome Entry Site) IRES, located in the 5’UTR region, is a cis-acting element which can attract the ribosome complexes to synthesize proteins. IRES is known to be involved in cell apoptosis and the regulation of oncogenes and growth factors. IREZone (Internal Ribosome Entry Zone), a region of multiple overlapping IRES structures, has been shown to have strong translational regulation capability. Therefore, knowing the information of IREZone or IRES in the 5’UTR of a gene is very helpful for studying the translational regulation mechanism of that gene. In this study, we use the Scan-For-Matches software to identify the IRES in the 5’UTR region of each human mRNA transcript. Then we define IREZone as a region with at least three overlapping IRES. uORF is the open reading frames locates 5’UTR upstream and most of them which involve in some important regulations of biological process belong to Oncogene. Ribosome profiling provides information of genetic synthesis in proteins. It can understand clearly relations between translational control and cis-acting element by means of combining analysis of ribosome profiling. Finally, we construct a database called mRNA 5’UTR translational regulation motifs, that is available online at http://cosbi2.ee.ncku.edu.tw/transbrowser/.