Synthesis、Characterization and Application of Au-Diamond nanohybrids in probing local thermal stability of membrane nanotube

• Main Authors: HUANG, JO-SHAN, 黃若姍
• Other Authors: WU, CHIH-CHE
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/9589hf

碩士 === 國立暨南國際大學 === 應用化學系 === 104 === Temperature is an important indicator for the health of living systems. Recent advances in luminescence thermometry have made it possible to detect the transient temperature change with high spatial resolution and sensitivity using fluorescent nanodiamond1. Here, we show that gold nanorod-fluorescent nanodiamond (GNR-FND) hybrid is produced as a two-in-one (heater & thermometer) nanoprobe to probe the local temperature and thermal structural stability. Polyarginine-coated FNDs were mixed with citrate-capped gold nanorods (GNRs) to form a dual-functional nanoprobe via multivalent electrostatic interactions. With the use of a 594 nm laser for both heating and probing, we are able to determine the transient temperature jumps by recording the spectral shifts of the zero-phonon lines of negatively charged nitrogen-vacancy centers in FNDs. A significant temperature jump (45±2K) induced by using differential Laser-induced heating on single GNR-FND was observed. The technique allowed us to quantitatively characterize the transition temperature (Tm) at which the intercellular membrane nanotube could break down. Our results demonstrates the capability of GNR-FND hybrid for efficient local heating and temperature sensing, which paves the way towards in vivo characterization of local thermal stability of cellular structures in all living things.