| Summary: | 碩士 === 國立暨南國際大學 === 應用化學系 === 104 === Several issues hurdle the analysis of bioamines. These limitations could be 1) the tiny amount in vivo, especially when the analysis targets are small such as fruit fly; 2) the involvement of artifacts in the extraction process. Current approaches for bioamine analysis include HPLE-ECD, HPLC-MS, GC-MS, CE-LIF, and cyclic voltammetry. These methods hold excellent analysis ability; however, asides from the high cost of the instruments, they also suffer the aforementioned limitations. Therefore, a cost effective, fast, convenient, low contamination, sensitive and accurate sensor system is needed for bioamine determination. In this study, a PDMS-based microfluidic chip device was developed for tyramine analysis. SU-8 photoresist was spin-coated on the wafer, which was subsequently underwent photo-lithography to make master pattern. PDMS was then poured onto the pattern to produce microfluidic chips. The microsturctuer was confirmed by scanning electron microscope(SEM). For surface modification, oxgen plasma was used to turn the surface hydrophobic methyl group to hydrophilic hydroxyl group. The chip system successfully determined fluorescein isothiocyanate (FITC) at alkaline condition (pH 8.5).
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