| Summary: | 碩士 === 國立交通大學 === 光電科技學程 === 104 === A microfluidic system has recently been developed and applied to fluorescence detection for biological characterization and quantitative chemical analysis. Because of the small amount of the microfluidic sample used, the fluorescence produced can be weak. Therefore, the major part of the microfluidic system involves optical components or complex optical equipment for improving fluorescence intensity detection efficiency, causing the system to be bulky. However, the complexity of the manufacturing process and high costs are common problems. In this study, a wireless probe for an improved microfluidic fluorescence detection system is proposed. Miniature fluorescent optical module integrated laser focusing and fluorescence collection was used to achieve the dual function and simplify the optical setup. In addition, polydimethylsiloxane materials were used for fabricating a multifunctional lens and reducing manufacturing costs, and a laser diode and photodiode were used for combining optical modules. The microfluidic fluorescence detection system has built-in wireless capabilities that reduce the number of transmission wires required and enhances the portability of the system. Finally, the experimental results demonstrated that the system satisfactorily enhanced Rhodamine 6G and Pacific Orange by fluorescence detection, and the overall increase in the collection of the fluorescence signal reached 150% without a lens. This system also controls a laser pulse, can operate in continuous excitation mode for performing fluorescence lifetime measurements, and has low complexity as well as a small equipment volume.
|
|---|
