Construction of a Microscope System with image revision procedure for two different scanning methods

• Main Authors: Hsu, Po-Chih, 許博智
• Other Authors: Liu, Jia-Ming
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/15897579427943183885

碩士 === 國立交通大學 === 影像與生醫光電研究所 === 104 === The microscope of our laboratory has laser scanning and confocal laser scanning modes, both based on nanopositioning stage system. To make the microscope system more versatile, we add a galvanometer scanning system, which has a much higher scanning speed than the nanopositioning stage scanning system. The microscope system is operated with a program coded in LabVIEW™. The program integrates optical elements, the nanopositioning stage, and the galvanometer scanning system, which are controlled by the program through a computer. There are mismatches in translation, rotation, and scaling between the images taken with the two scanning systems. In this study, we develop a procedure to properly match the images taken with the two scanning systems through post proceeding revision using cross correlation and phase correlation schemes.