A Study of Synchronized Burst Mechanisms in Neuronal Cultures
博士 === 國立中央大學 === 物理學系 === 104 === Synchronized bursting (SB) is a general spontaneous activity of neuronal cultures, however, their origin is still unclear. Here, we investigate the properties of these SBs in a culture on multi-electrode array (MEA) system. We find that characteristics of these SBs...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | en_US |
| Published: |
2016
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/3dww22 |
| Summary: | 博士 === 國立中央大學 === 物理學系 === 104 === Synchronized bursting (SB) is a general spontaneous activity of neuronal cultures, however, their origin is still unclear. Here, we investigate the properties of these SBs in a culture on multi-electrode array (MEA) system. We find that characteristics of these SBs can be used to represent the different developmental stages of the cultures and these characteristics can be modified by pharmacological treatments. The spatiotemporal property of SBs and its reverberations are studied by a high-resolution MEA containing 4096 electrodes. The result indicates that SB fires with preferred pathways rather than completely random generation. Furthermore, we produce a neuronal cultures with different amounts of glial cells to study the effects of glia on SBs. The finding indicates that glia are interacting with neurons in the network to coordinate the firings. A mean-field model based on short term synaptic plasticity and recurrent connections has been developed to understand these characteristics. A phase diagram obtained from this model shows that networks exhibiting SBs are in an oscillatory state due to large enough positive feedback provided by synaptic facilitation and recurrent connections. Our finding suggests that networks with SBs have excessive recurrent connections and might have very little information processing capabilities.
|
|---|