| Summary: | 碩士 === 國立嘉義大學 === 動物科學系研究所 === 104 === This study was divided into three parts. The first part: Screening of cellulolytic microorganism from 24 strains of Ascomycota, Basidiomycota and Zygomycota. Results showed that the growth rate of Trichoderma. reesei 1-1, 1-2, QM6a, T. viride, T. virens FT333, Aspergillus niger, Aspergillus flavus, Penicillium aculeatum, Botryosphaeria rhodina RCYU30101, Cordyceps militaris, Volvariella volvacea, Chaetomium globsum and Rhizopus sp. were better than others. And then compared in the Mandels-Reese agar plate. We found that Aspergillus niger(AN), Aspergillus flavus(AF), Penicillium aculeatum(PA), T. virens FT333(TV), Trichoderma. reesei QM6a(TR) and Volvariella volvacea (VV) had the highest diameter of clear zone. The second part: Establishing conditions of solid-state fermentation, to achieve the best activity of enzymes. Results showed that 6 strains using wheat bran as substrate, adjusting moisture to 70% and fermented for 5 days, each one can achieve the best growth condition. The third part: According to the result of part one and part two, producing 6 cellulolytic microorganisms fermented product. Investigate the effect of dietary supplementation of cellulolytic microorganism fermented product on growth performance, carcass traits and intestinal physiology in broilers, to evaluate whether it can replaced commercial composite enzymes or not. A total of 320 one-day-old female broiler chickens were assigned to 8 different treatments: Control group, 0.025% commercial enzymes group; 0.1% fermented product of AN, AF, PA, TV, TR and VV group. Each treatment was divided into 4 replicates of 10 birds for 38 days experiment. Results showed that AF were significantly higher on body weight in day-28 (P<0.05), AN were significantly higher on feed conversion ratio (FCR) in whole experiment. The performance efficiency factors (PEF) on AN were significantly higher in whole experiment (P<0.05).In intestinal physiology, the total volatile fatty acid (VFA) of caecum content of AF were significantly higher than others (P<0.05). In summary, AN and AF fermented product has the potential to be developed into the feed additive in broilers.
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