| Summary: | 碩士 === 國立宜蘭大學 === 生物資源學院碩士在職專班 === 104 === Background: spinal cord injury (SCI) is a damage, caused by external force, spinal cord (SC) disease, and aging or degeneration, which induces oxidative stress, to SC that affects its function. Human CISD2 gene is located on chromosome 4, which is relative to longevity. Previous reports have demonstrated that degeneration of neuron, muscle, as well as aging is observed in CISD2 knockout mice, indicating the role of CISD2 gene in neuron protection. Curcumin is a main component of turmeric and has effects on anti-oxidation, anti-inflammation and neuron protection. We previously have found that CISD2 regulates apoptosis and inflammation of neuron, and curcumin regulates expression of CISD2. This study further investigates 1) how CISD2 regulates affects structure and oxidative damage in neuron and 2) how curcumin modulates expression of CISD2 to achieve neuron protection.
Materials and methods: Several physiological parameters are detected after siRNA-mediated CISD2 knockdown, LPS-induced inflammation and/or curcumin treatment in cultures of neuron-like SH-SY5Y cells.
Results: Under LPS-induced cell injury model to mimic conditons of SCI, CISD2-knockdown cells manifest 1) decreased ROS accumulation and altered mitochondria membrane potential after curcumin treatment and 2) decreased apoptosis and increased survival by curcumin treatment.
Conclusion: 1) CISD2 expression alleviates increment of mitochondria membrane potential and ROS in neural cells sustained LPS-induced and 2) curcumin upregulates CISD2 expression and attenuates injury–triggered mitochondrial dysfunction in in vitro/ injury model in a neuron inflammation model.
|
|---|
