| Summary: | 碩士 === 國立高雄海洋科技大學 === 水產食品科學研究所 === 104 === Previous studies in our laboratory found that eel protein hydrolysate (EPH) obtained from autoclave and proteinase hydrolysis displays the in vitro antioxidant, hypotensive and anticoagulant activities. In this study, response surface methodology (RSM), central composite design (CCD), was used to investigate the effect of hydrolysis time (HT) and enzyme to substrate ratio (E/S) on the amino group concentration (degree of hydrolysis), inhibitory ACE activity, and DPPH scavenging of various hydrolyzed products. The results show that E/S and HT have significant impact on the amino group concentration, inhibitory ACE activity, and DPPH scavenging effect (p < 0.01). Moreover, the lack of fit of the RSM models are not significant (p > 0.05). The highest amino group concentration of the hydrolysates is 389.1 ± 0.7 μg/mL (A4). The best performance in the inhibition of ACE activity and DPPH radical scavenging effect is P8 hydrolysate, its efficacy of 71.2 ± 0.1%, 63.55 ± 0.79%, respectively. P8 hydrolysate on HepG2 cell is non-toxic and it has a protective effect on HepG2 oxidative damage induced by H2O2. In addition, P8 also demonstrates a protective effect on plasmid DNA oxidative damage induced by H2O2. In animal studies, a hypotensive effect on the spontaneously hypertensive rat (SHR) was observed by P8 hydrolysate treatments.
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