1H-NMR-based metabolomic analysis of oviparitine and ovoviviparitine reproduction in brine shrimp

• Main Authors: Bo-Hua Yu, 余柏樺
• Other Authors: Kuo-Hsun Chiu
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/98142071389882794177

碩士 === 國立高雄海洋科技大學 === 水產養殖研究所 === 104 === There are two different ways in Artemia breeding strategies, oviparity and ovoviviparity. Nowadays, many studies had demonstrated that the diapause eggshell of Artemia is necessary for oviparity to successful survival in the extreme environments, but the discrepancy of mechanism between oviparous and ovoviviparous is still unclear. In order to reveal the differences, two ways of Artemia breeding strategies are analyzed through NMR techniques, Metabolomics. The result suggested that several metabolites including 5,6-dihydrouracil, betaine, malate, methylamine, methylguanidine, N-acetylaspartate, succinate, trimethylamine and β-alanine are significantly different between oviparous and ovoviviparous stages. The analysis of the Principal Component Analysis (PCA) and Partial Least Squares-Discriminant Analysis (PLS-DA) also strongly indicated that the differences between oviparity and ovoviviparity are significant. The consequences of Loading bi plot showed that the nine metabolites involved in ovoviviparity are very important. Taken together, Artemia under extreme environment may initiate different metabolism, including β-alanine and pyrimidine metabolism, pantothenate and co-A biosynthesis and citrate cycle (TCA cycle) these pathway.