| Summary: | 碩士 === 國立屏東科技大學 === 生物科技系所 === 104 === MicroRNAs (miRNAs) are 20-22 nucleotides noncoding RNAs that have been identified in various organisms. In plants, miRNAs are mostly involved in the regulation of growth and developmental processes such as leaf and root development, phase transitions, and flowering. In Arabidopsis , microRNA160 (miR160) negatively regulates three auxin response factor (ARF) genes (ARF10/ARF16/ARF17) and therefore controls several developmental processes, including primary and lateral root growth. In Arabidopsis , miR160 can regulate expression of ARF. In this study, a nature species Phalaenopsis amabilis, was selected to study the expression of gene regulation of miR160. First, useing rapid-amplification of cDNA ends (RACE) to get the full-length sequence of the gene, ARF10 has 1,935 bp, and ARF16 with 2,052 bp. Second, miR160 in different expression of different tissues, we can find that miR160 exists in all tissues of Phalaenopsis amabilis, and the level of flower stalk is higher than the other tissues. In semi-quantitative RT-PCR analyses of ARF10 and ARF16 genes of total RNA from different tissue of Phalaenopsis amabilis, and the result shows that all tissues except
for flower stalk have full-length gene. From the above results, ARF10 and ARF16 gene of Phalaenopsis amabilis are targeted by miR160, and miR160 will cleavge the target gene and gene expression will be repressed.
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