Antihypertensive Effect of Novel Angiotensin I Converting Enzyme Inhibitory Peptides Derived From Enzymatic Hydrolysis of Salmon Protamine
碩士 === 國立屏東科技大學 === 生物科技系所 === 104 === Angiotensin I converting enzyme (ACE) inhibitory peptide is widely recognized as possible cures for hypertension. Bioactive peptides from natural sources, including marine fish, are more considered because it has no harm effects. The objective of this study is...
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ndltd-TW-104NPUS51110202017-01-01T04:05:30Z http://ndltd.ncl.edu.tw/handle/02344255779417384070 Antihypertensive Effect of Novel Angiotensin I Converting Enzyme Inhibitory Peptides Derived From Enzymatic Hydrolysis of Salmon Protamine 自鮭魚精蛋白酵素水解物分離純化具有血管收縮素轉化酶抑制活性之胜肽 Fikriyah Rasyad 裴莉亞 碩士 國立屏東科技大學 生物科技系所 104 Angiotensin I converting enzyme (ACE) inhibitory peptide is widely recognized as possible cures for hypertension. Bioactive peptides from natural sources, including marine fish, are more considered because it has no harm effects. The objective of this study is screeningthe presence of potential ACE inhibitory peptide from salmon protamine. ACE inhibitory peptide was obtained from salmon protamine after 16 hours of hydrolysis by various enzymes and followed by ultrafiltration on a 3 kDa molecular weight cut off (MWCO) membrane. Their sequences were analysed by Liquid Chromatography Tandem Mass Spectrometric (LC-MS/MS). ACE inhibitory activity was measured using Reverse Phase High Performa Liquid Chromatography (RP-HPLC).The most potential ACE inhibitory peptide was synthesized using a method of Solid Phase Peptide Synthesis. The results indicated that trypsin hydrolysate had the highest ACE inhibitory activity compared to the other hydrolysates with the IC50 value 135.96 µg/ml. LC-MS/MS analysis of tryptic protamine identified two major peaks with three peptide sequences, Ser-Ser-Arg-Pro-Ile-Arg (SR-6), Ser-Ser-Ser-Arg-Pro-Ile-Arg (SR-7), Pro-Arg-Arg-Ala-Ser-Arg (PR-6) which sourced from salmine of Chum Salmon (Oncorhynchus keta). Furthermore these two peakswere fractionated and the ACE inhibitory activity assay showed that fraction 2, belongs to SR-6 and SR-7 peptides, has the highest activity. IC50 value of peptide SR-7 was 94.98 ± 2.63 µM. Therefore, it can be concluded that salmon protamine has ACE inhibitory peptides and it may be beneficial for preventing hypertension and can be used as functional food ingredients. Tzou-Chi Huang 黃卓治 2015 學位論文 ; thesis 56 en_US |
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碩士 === 國立屏東科技大學 === 生物科技系所 === 104 === Angiotensin I converting enzyme (ACE) inhibitory peptide is widely recognized as possible cures for hypertension. Bioactive peptides from natural sources, including marine fish, are more considered because it has no harm effects. The objective of this study is screeningthe presence of potential ACE inhibitory peptide from salmon protamine. ACE inhibitory peptide was obtained from salmon protamine after 16 hours of hydrolysis by various enzymes and followed by ultrafiltration on a 3 kDa molecular weight cut off (MWCO) membrane. Their sequences were analysed by Liquid Chromatography Tandem Mass Spectrometric (LC-MS/MS). ACE inhibitory activity was measured using Reverse Phase High Performa Liquid Chromatography (RP-HPLC).The most potential ACE inhibitory peptide was synthesized using a method of Solid Phase Peptide Synthesis. The results indicated that trypsin hydrolysate had the highest ACE inhibitory activity compared to the other hydrolysates with the IC50 value 135.96 µg/ml. LC-MS/MS analysis of tryptic protamine identified two major peaks with three peptide sequences, Ser-Ser-Arg-Pro-Ile-Arg (SR-6), Ser-Ser-Ser-Arg-Pro-Ile-Arg (SR-7), Pro-Arg-Arg-Ala-Ser-Arg (PR-6) which sourced from salmine of Chum Salmon (Oncorhynchus keta). Furthermore these two peakswere fractionated and the ACE inhibitory activity assay showed that fraction 2, belongs to SR-6 and SR-7 peptides, has the highest activity. IC50 value of peptide SR-7 was 94.98 ± 2.63 µM. Therefore, it can be concluded that salmon protamine has ACE inhibitory peptides and it may be beneficial for preventing hypertension and can be used as functional food ingredients.
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author2 |
Tzou-Chi Huang |
author_facet |
Tzou-Chi Huang Fikriyah Rasyad 裴莉亞 |
author |
Fikriyah Rasyad 裴莉亞 |
spellingShingle |
Fikriyah Rasyad 裴莉亞 Antihypertensive Effect of Novel Angiotensin I Converting Enzyme Inhibitory Peptides Derived From Enzymatic Hydrolysis of Salmon Protamine |
author_sort |
Fikriyah Rasyad |
title |
Antihypertensive Effect of Novel Angiotensin I Converting Enzyme Inhibitory Peptides Derived From Enzymatic Hydrolysis of Salmon Protamine |
title_short |
Antihypertensive Effect of Novel Angiotensin I Converting Enzyme Inhibitory Peptides Derived From Enzymatic Hydrolysis of Salmon Protamine |
title_full |
Antihypertensive Effect of Novel Angiotensin I Converting Enzyme Inhibitory Peptides Derived From Enzymatic Hydrolysis of Salmon Protamine |
title_fullStr |
Antihypertensive Effect of Novel Angiotensin I Converting Enzyme Inhibitory Peptides Derived From Enzymatic Hydrolysis of Salmon Protamine |
title_full_unstemmed |
Antihypertensive Effect of Novel Angiotensin I Converting Enzyme Inhibitory Peptides Derived From Enzymatic Hydrolysis of Salmon Protamine |
title_sort |
antihypertensive effect of novel angiotensin i converting enzyme inhibitory peptides derived from enzymatic hydrolysis of salmon protamine |
publishDate |
2015 |
url |
http://ndltd.ncl.edu.tw/handle/02344255779417384070 |
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