| Summary: | 碩士 === 國立屏東科技大學 === 動物疫苗科技研究所 === 104 === Streptococcosis in cultured fish initiated by Streptococcus iniae has resulted in high losses in the aquaculture industry. In addition, S. iniae is also an important zoonotic microbe to infect humans and cause severe bacteremia cellulitis. According to the proteomic results in our previous studies, we selected two proteins, GAPDH and SOD, to become vaccine candidates for further investigation. In the present study, we extended the previous findings to develop an effective fish vaccine against S. iniae based on GAPDH and SOD. GAPDH gene and SOD gene were respectively cloned to express rGAPDH (37.84 kDa) and rSOD (23.1 kDa) by E. coli system. Four groups of 30 fish each were intraperitoneally injected twice at a 14-day interval with PBS, rSOD (50 g/fish), rGAPDH (50 g/fish) or rGAPDH (50 g/fish) plus rSOD (50 g/fish). All vaccine regimens emulsified with Montanide ISA 763A at a 1:1 ratio (v/v). In comparison with PBS group, the GAPDH vaccination group showed 45.45% relative percent survival (RPS), the SOD vaccination group showed 68.16% RPS and the vaccination GAPDH plus SOD group displayed 86.36% RPS. In conclusion, the survival rates indicate the feasibility of these two subunit vaccine candidates to be developed as effective subunit vaccines against S. iniae. Moreover, GAPDH sequences of the genus Streptococcus are quite similar to employ as a universal vaccine for prevention of complicated streptococcosis in the future.
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