Detection of human papillomavirus in squamous cell carcinoma arising from dermoid cysts

• Main Authors: An-Jen Chiang, 蔣安仁
• Other Authors: Tsung-Hsien Chang
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/01685112301468737093

博士 === 國立中山大學 === 生物科學系研究所 === 104 === Primary squamous cell carcinoma (SCC) of the ovary in humans is rare. Most cases represent a malignant transformation of ovarian teratoma, Brenner tumor, or endometriosis. The etiology of this cancer remains largely unknown. Human papillomavirus (HPV) infection is a critical factor that induces tumor formation of cervical cancer. Based on the above findings, this study aimed to evaluate the association of HPV with malignant transformation of mature cystic teratoma (MCT) into the SCC of ovary. The specimens used in this study included formalin-fixed paraffin-embedded SCC-MCT tissues and their adjacent tissues from the cervix to ovaries of four patients. A total 12 cases of benign teratoma ovarian tissues (dermoid tissues) and 11 cases of benign non-teratoma ovarian tissues (non-dermoid tissues) were collected and examined. Two squamous carcinoma tissues of the cervix were used as control samples. HPV was detected by immunohistochemistry (IHC) with antibodies against either HPV capsid or E6 of HPV type 16/18, and by in situ hybridization (ISH) with three sets of genotyping probes of HPV types 6/11, 16/18 and 31/33. The results of IHC analysis revealed the HPV infection in the four cases of SCC-MCT and the two control cervical cancer samples. Importantly, HPV was also detected in adjacent reproductive tissues of the SCC-MCT cases, suggesting that viral particles might spread through an ascending tissue route from the fallopian tubes, endometrium, endocervix and cervix to the ovaries. ISH results revealed the presence of HPV type 16/18 in all SCC-MCT cases and HPV type 31/33 in two, with no HPV type 6/11 in any SCC-MCT cases. However, when compared with the SCC-MCT cases, the lower detection rates of HPV in dermoid cysts and nondermoid tissues suggested that HPV viral infection might not be associated with normal ovarian tissues or benign ovarian teratomas.