Highly Efficient Tumor-Targeted Nanoparticles Deliver TRAIL as Gene Therapy For Hepatocellular Carcinoma
碩士 === 國立清華大學 === 生物醫學工程研究所 === 104 === Gene therapy for cancer treatment has become the very promising approach of hepatocellular carcinoma (HCC). However, the therapeutic plasmid DNA delivery into cancer cell and regulate the expression is a challenge of many studies. We utilize SP94 peptide as ta...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2016
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| Online Access: | http://ndltd.ncl.edu.tw/handle/62327978846859283295 |
| Summary: | 碩士 === 國立清華大學 === 生物醫學工程研究所 === 104 === Gene therapy for cancer treatment has become the very promising approach of hepatocellular carcinoma (HCC). However, the therapeutic plasmid DNA delivery into cancer cell and regulate the expression is a challenge of many studies. We utilize SP94 peptide as targeted ligand modifies liposome calcium phosphate nanoparticles (LCP-NPs) to specifically target HCC. We also use protamine to improve transfection efficiency which contains several nuclear localization signal (NLS). Moreover, we want to deliver an apoptosis inducer gene -TRAIL (Tumor necrosis factor-related apoptosis-inducing ligand) to induce tumor cell apoptosis. These results demonstrated that targeting LCP-NPs can specifically delivery therapeutic plasmid DNA into HCC to regulate cancer cell expression.
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